Table 2.
Constituents and concentrations of the various simulated juices used in the in vitro digestion model.∗
| Components | Saliva | Gastric juice | Duodenal juice | Bile juice |
|---|---|---|---|---|
| Inorganic components | 10 ml KCl 89.6 g/l 10 ml KSCN 20 g/l 10 ml NaH2PO4 88.8 g/l 10 ml Na2SO4 57 g/l 1.7 ml NaCl 175.3 g/l 20 ml NaHCO3 84.7 g/l |
15.7 ml NaCl 175.3 g/l 3.0 ml NaH2Po4 88.8 g/l 9.2 ml KCl 89.6 g/l 18 ml CaCl2.2 H2O 22.2 g/l 10 ml NH4Cl 30.6 g/l 6.3 ml HCl 37 % g/g |
40 ml NaCl 175.3 g/l 40 ml NaHCO3 84.7 g/l 10 ml KH2PO4 8 g/l 6.3 ml Kci 89.6 g/l 10 ml MgCl2 5 g/l 180 μl HCl 37% g/g |
30 ml NaCl 175.3 g/l 68.3 ml NaHCO3 84.7 g/l 4.2 ml KCl 89.6 g/l 150 μl HCL 37 % g/g |
| Organic components | 8 ml urea 25 g/l | 10 ml glucose 65 g/l 10 ml glucuronic acid 2 g/l 3.4 ml urea 25 g/l 10 ml glucosamine hydrochloride 33 g/l |
4 ml urea 25 g/l | 10 ml urea 25 g/l |
| Add to the mixture of organic + inorganic components | 290 mg α-amylase 2.5 g pepsin 25 mg mucin |
1 g BSA 15 mg uric acid 3 g mucin |
9ml CaCl2.2H2O 22,2 g/l 1 g BSA 9 g pancreatin 1.5 g lipase |
10 ml CaCL2.2H2O 22.2 g/l 1.8 g BSA 30 Embed |