Figure 2.

Oxidative challenge upregulates transcriptional levels of glycogen synthesis components in BME26 cells. Transcription of (A) glycogen synthase (GS), (B) glycogen synthase kinase 3 β (GSK3β), (C) phosphoglucomutase (PGM), and (D) glycogen debranching enzyme (GDE) in Rhipicephalus microplus embryonic cell line (BME26) was measured 2 and 24 h after addition of H₂O₂ (2 or 4 mM). E, diagram of glycogen metabolism depicting pathways involved in glycogen synthesis and degradation. Experiments were performed in three independent biological samples with three experimental replicates each, where ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, compared with the control; and #p < 0.05, ####p < 0.0001, compared between the groups, in Tukey’s multiple comparisons test.