Figure 6.

Decrease in mitochondrial respiratory rates induced by H₂O₂treatment on BME26 cells.A, typical traces of oxygen consumption rates (OCR, red line) and oxygen concentration (blue line) of BME26 cells after treatment with 2 and 4 mM H₂O₂ for 2 h. B, oxygen consumption rates from BME26 cells (open circle), and after treatment with 2 (open triangle up) or 4 mM (triangle closed down) H₂O₂ for 2 h. Data are expressed as mean ± standard deviation (SD) of five different experiments. Comparisons between groups were done by two-way ANOVA for repeated measurements and a posteriori Holm–Sidak test, adjusted for multiple comparisons (p < 0.001). C, fluorescence images of mitochondrial membrane of BME26 cells stained with 200 nM MitoTracker Green (λ ex = 488 nm, λ em = 500–530 nm) after 2-h oxidative challenge. Yellow boxes indicate a digital zoom of one cell. Yellow arrow shows mitochondria elongated shape, whereas white arrows show dotted mitochondria shape. The scale bar represents 100 μm.