Antibodies
| Target | Source/catalog number | Application/species/dilution |
|---|---|---|
| anti-METTL5 | ProteinTech/16791-1-AP | Western blot/rabbit/1:500 dilution |
| anti-METTL5 | Atlas/SAB2101471 | IF/rabbit/1:250 dilution |
| anti-TRMT112 | Santa Cruz Biotechnology/sc-398481 | Western blot/mouse/1:100 dilution |
| anti-FLAG-M2 | Sigma/A8592 | Western blot/mouse/1:10,000 dilution |
| anti-RPL4 | Proteintech/11302-1-AP | Western blot/rabbit/1:1000 dilution |
| anti-RPS6 | Abcam/70227 | Western blot/rabbit/1:1000 dilution |
| anti-EIF3A | Cell Signaling Technology/3411 | Western blot/rabbit/1:1000 dilution |
| anti-EIF4E | Cell Signaling Technology/2067 | Western blot/rabbit/1:1000 dilution |
| anti-GAPDH-HRP | GenScript/A00192-100 | Western blot/goat/1:10,000 dilution |
| anti-SNRP70 | Abcam/ab83306 | Western blot/rabbit/1:1000 dilution |
| anti-histone H3 | Cell Signaling Technology/D1H2 | Western blot/rabbit/1:2000 dilution |
| anti-fibrillarin | Abcam/ab4566 | IF/mouse/1:250 dilution |
| goat-anti-rabbit-488 | Thermo Fisher/A11008 | IF secondary, 1:500 dilution |
| goat-anti-mouse-594 | Thermo Fisher/A11005 | IF secondary, 1:500 dilution |
| goat anti-rabbit HRP | Thermo Fisher/A16110 | Western blot, secondary, 1:5000 dilution |
| goat anti-mouse HRP | Thermo Fisher/A16078 | Western blot, secondary, 1:5000 dilution |
| donkey-anti-rabbit DyLight 800 | Thermo Fisher/SA5-10044 | Western blot, secondary, 1:5000 dilution |
| donkey-anti-rabbit DyLight 680 | Thermo Fisher/SA5-10170 | Western blot, secondary, 1:5000 dilution |