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. 2022 Jan 25;298(3):101636. doi: 10.1016/j.jbc.2022.101636

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

The negatively charged surface of AcrIF13 is involved in Csy binding.A, electrostatic surface representation of AcrIF13. The negatively charged residues which are mutated in mutagenesis assays are marked. B, MST assay of the binding between the Csy complex and AcrIF13 or its mutants. The binding curves of the six AcrIF13 mutants with binding K_D values of over 10-fold higher than that of WT AcrIF13 are displayed. Error bars represent SD; n = 3. Acr, anti-CRISPR; Csy, CRISPR-Cas surveillance complex; MST, microscale thermophoresis.