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EMSA results of AcrIF13 and its mutants.A–C, EMSA experiments using Csy complex, target DNA, and AcrIF13 or its mutants. DNA binding by Csy complex produced a shift of the target DNA. Addition of gradually increasing concentrations of AcrIF13 or its mutants would decrease the shifted DNA to different extents. Acr, anti-CRISPR; Csy, CRISPR-Cas surveillance complex; EMSA, electrophoretic mobility shift assay.
