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PSP94 inhibits sterol binding by CAP proteins.A–C, sterol binding by CAP family members in the absence (A) or presence of PSP94 (B) was assessed by microscale thermophoresis. CAP family members bound cholesterol sulfate in the micromolar range, but this binding is blocked in the presence of PSP94, which by itself does not bind cholesterol sulfate (C). Measurements were performed in triplicates, and the corresponding dissociation constants (Kd) are indicated. N/A, not applicable.
