Figure 5.

Reactive astrocytes and scar-forming astrocytes were affected by transplanted NESCs. (a) Spinal cord sections of lesion sites from vehicle treated rats in chronic transplantation condition showing a large cyst surrounded by parenchyma stained for GFAP (green) and S100A10 (red). (b) Spinal cord sections of lesion site from vehicle and NESCs groups after chronic transplantation stained for HuNu (green), CSPG4 (red) and DAPI (blue). (c) Spinal cord sections of lesion sites stained for HuNu (green) and GFAP (red) from both vehicle and NESCs groups 10 weeks after subacute or chronic transplantation. (d) Quantification of the GFAP positive area to whole section of the experiment illustrated in b (mean ± SEM, two-tailed t-test, subacute: p < 0·01, n = 5 and 6 rats, respectively, chronic: no statistical significance, n = 6). (e) Spinal cord sections of lesion sites from vehicle and NESCs groups 10 weeks after subacute or chronic transplantation stained for ED1. (f) Magnified image of the boxed area in e. (g) Quantification of ED1 positive cells 10 weeks after subacute or chronic transplantation as described in E (mean ± SEM, two-tailed t-test, subacute: p < 0·05, n = 5 and 9 rats, respectively, chronic: p < 0·05, n = 7 rats per group). Section thickness = 10 µm.