Fig. 7.

Activation of p38 and ERK1/2 pathways in response to stretch. Phosphorylation levels of p38 proteins in stretched and control (unstretched) cells measured after 20 min of stretch (A) and 24 h of stretch (C). Phosphorylation levels of ERK proteins in stretched and control cells measured at 20 min (B) and 24 h (D). Asterisks (*) indicate a significant effect of stretch on MAPK phosphorylation (P < 0.05). Open triangles (Δ) signify individual control (unstretched) data points, and open circles (○) are individual data points from stretched cells. Points with similar numerical values were staggered for better readability. Error bars represent standard error of the mean of each group. N = 3 for each group. The n-value represents a fibroblast line from the same individual fish maintained in separate passages, cryopreserved on a different passage number and day, and thawed for experiments on different days. Figure modified from (Johnston and Gillis, 2020).