Fig. 8.

The effect of miR-29b transfection on collagen type I protein levels. Cardiac fibroblast cultures were transfected with 10 nmol l⁻¹ mature zebrafish miR-29b mimic or a nonsense small interfering RNA (siRNA), and re-transfected after 3 days, with sampling occurring at 7 days. Mean collagen type I levels measured by densitometry and standardized to total protein on the same membrane, which was cut in half and blotted separately. Letters represent a significant change relative to control (P < 0.05). n = 3 for each experiment, with each n being a protein sample extracted from cultured cells derived from the same trout ventricle maintained in separate passages and cryovials, and thawed on different days, as previously described (Johnston and Gillis, 2017). Figure modified from (Johnston et al., 2019).