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. 2022 Feb 15;3(1):101178. doi: 10.1016/j.xpro.2022.101178
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CK19 Developmental Studies Hybridoma Bank (DSHB) Cat.no.TROMAIII
CEACAM1 LSBio Cat.no. LS-C106710
GS (Glutamine synthetase) Sigma-Aldrich Cat.no.G2781

Chemicals, peptides, and recombinant proteins

Sodium phosphate monobasic monohydrate Sigma-Aldrich Cat.no. S9638
Sodium phosphate dibasic (anhydrous) Sigma-Aldrich Cat.no. S9763
Sodium azide Sigma-Aldrich Cat.no. S2002
16% w/v Paraformaldehyde Fisher Scientific Cat.no. 50-980-487
2% bio-acrylamide Bio-Rad Cat.no. 161-0142
40% acrylamide Bio-Rad Cat.no. 161-0140
VA-044 Wako Chemicals, Fisher Cat.no. NC0632395
X-CLARITYTM Electrophoretic Tissue Clearing Solution Logos Bio Cat.no. C13001
N,N,N′,N′ Tetrakis(2-Hydroxypropyl)ethylenediamine (Quadrol) Sigma-Aldrich Cat.no. 122262
Boric acid Fisher Scientific Cat.no. A74-500
Sodium sulfite Sigma-Aldrich Cat.no. S0505
Bovine serum albumin (BSA) Fisher Scientific Cat.no. BP1605-100
Sodium dodecyl sulfate (SDS) Sigma-Aldrich Cat.no. L3771
Triton X-100 Fisher Scientific Cat.no. BP151-500
Urea Sigma-Aldrich Cat.no. U5378
Sucrose Sigma-Aldrich Cat.no. S9378
Ammonium hydroxide 25% Sigma-Aldrich Cat.no. 105432
30% Hydrogen peroxide Fisher Scientific Cat.no. H325-500

Experimental models: Organisms/strains

Wild type mice, C57BL6 The Jackson Laboratory N/A
Mouse: Foxa3-Cre YAP1 KO [Tg(Foxa3-Cre)1Khk, Yap1tm1.1Dupa, Gt(ROSA)26Sortm1(EYFP)Cos/J] Molina et al., (2021) N/A

Software and algorithms

Imaris 9.7.2 Bitplane N/A
Alternative image process and analysis software

Other

50 mL Falcon conical tubes Fisher Scientific Cat.no. 05-539-9
X-Clarity clearing system (this study) LogosBio Cat.no. XC-ETC 2
Alternative clearing systems See other resources section N/A
Sample holder for X-Clarity clearing system (3D printed) See other resources section N/A
Ribbon Scanning Confocal (this study) Caliber I.D. Cat.no. RS-G4
Alternative cleared tissue microscopy imaging platform