| Antibodies |
|
| CK19 |
Developmental Studies Hybridoma Bank (DSHB) |
Cat.no.TROMAIII |
| CEACAM1 |
LSBio |
Cat.no. LS-C106710 |
| GS (Glutamine synthetase) |
Sigma-Aldrich |
Cat.no.G2781 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Sodium phosphate monobasic monohydrate |
Sigma-Aldrich |
Cat.no. S9638 |
| Sodium phosphate dibasic (anhydrous) |
Sigma-Aldrich |
Cat.no. S9763 |
| Sodium azide |
Sigma-Aldrich |
Cat.no. S2002 |
| 16% w/v Paraformaldehyde |
Fisher Scientific |
Cat.no. 50-980-487 |
| 2% bio-acrylamide |
Bio-Rad |
Cat.no. 161-0142 |
| 40% acrylamide |
Bio-Rad |
Cat.no. 161-0140 |
| VA-044 |
Wako Chemicals, Fisher |
Cat.no. NC0632395 |
| X-CLARITYTM Electrophoretic Tissue Clearing Solution |
Logos Bio |
Cat.no. C13001
|
| N,N,N′,N′ Tetrakis(2-Hydroxypropyl)ethylenediamine (Quadrol) |
Sigma-Aldrich |
Cat.no. 122262 |
| Boric acid |
Fisher Scientific |
Cat.no. A74-500 |
| Sodium sulfite |
Sigma-Aldrich |
Cat.no. S0505 |
| Bovine serum albumin (BSA) |
Fisher Scientific |
Cat.no. BP1605-100 |
| Sodium dodecyl sulfate (SDS) |
Sigma-Aldrich |
Cat.no. L3771 |
| Triton X-100 |
Fisher Scientific |
Cat.no. BP151-500 |
| Urea |
Sigma-Aldrich |
Cat.no. U5378 |
| Sucrose |
Sigma-Aldrich |
Cat.no. S9378 |
| Ammonium hydroxide 25% |
Sigma-Aldrich |
Cat.no. 105432 |
| 30% Hydrogen peroxide |
Fisher Scientific |
Cat.no. H325-500 |
|
| Experimental models: Organisms/strains |
|
| Wild type mice, C57BL6 |
The Jackson Laboratory |
N/A |
| Mouse: Foxa3-Cre YAP1 KO [Tg(Foxa3-Cre)1Khk, Yap1tm1.1Dupa, Gt(ROSA)26Sortm1(EYFP)Cos/J] |
Molina et al., (2021) |
N/A |
|
| Software and algorithms |
|
| Imaris 9.7.2 |
Bitplane |
N/A |
| Alternative image process and analysis software |
|
|
|
| Other |
|
| 50 mL Falcon conical tubes |
Fisher Scientific |
Cat.no. 05-539-9 |
| X-Clarity clearing system (this study) |
LogosBio |
Cat.no. XC-ETC 2 |
| Alternative clearing systems |
See other resources section |
N/A |
| Sample holder for X-Clarity clearing system (3D printed) |
See other resources section |
N/A |
| Ribbon Scanning Confocal (this study) |
Caliber I.D. |
Cat.no. RS-G4 |
| Alternative cleared tissue microscopy imaging platform |
|
|