Hydrogel Solution

Reagent	Final concentration	Amount
2% bioacrylamide (cold)	0.05%	1.25 mL
40% acrylamide (cold)	4%	5 mL
1× PBS (cold)	n/a	43.75 mL
VA-044	0.541 mM	0.175 g
Total	n/a	50 mL

Storage: Best used fresh but can be kept at 4°C for a week.