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| Reagent | Final concentration | Amount |
|---|---|---|
| Collagenase type V | 0.6 mg/mL | 0.03 g |
| Bovine serum albumin (BSA) | 0.02 g/mL | 1 g |
| RPMI media with PS | n/a | 50 mL |
| Total | n/a | 50 mL |
Collagenase should be stored at −20°C for 24 months. BSA should be stored at 4°C for 24 months. RPMI media should be stored at 4°C for 6 months. Collagenase solution prepared should be stored at −20°C for 6 months.
