REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Human: Islets from males aged 30–53 years old	Islet Core and Clinical Islet Laboratory (Alberta Islet Distribution Program, University of Alberta) and Multi-Organ Transplant/University Health Network Islet Isolation Program (University Health Network, Toronto, Ontario, Canada)	N/A
Chemicals, peptides, and recombinant proteins
D-Glucose	Sigma-Aldrich	Cat# 50-99-7
Emla cream	Aspen	900394
50% dextrose solution	Hospira	06648
Roswell park memorial institute (RPMI) 1640 media	Sigma-Aldrich	R8758
Fetal bovine serum (FBS)	Sigma-Aldrich	F1051
Penicillin-Streptomycin (PS)	Sigma-Aldrich	P4333
Collagenase type V	Sigma-Aldrich	C9263
Bovine serum albumin (BSA)	Sigma-Aldrich	A7906
Isoflurane	Fresenius Kabi	CP0406V2
Dulbecco's phosphate-buffered saline (DPBS)	Gibco	14190-144
Dithizone	Sigma-Aldrich	D5130
Dimethyl sulfoxide	Sigma-Aldrich	276855
Sodium chloride (NaCl)	BioShop	CAS# 7647-14-5
Potassium chloride (KCl)	BioShop	CAS# 7447-40-7
Potassium phosphate monobasic (KH2PO4)	BioShop	CAS# 7778-77-0
Magnesium sulfate (MgSO4)	BioShop	CAS# 7487-88-9
Calcium chloride dihydrate (CaCl2)	BioShop	CAS# 10035-04-8
Sodium bicarbonate (NaHCO3)	BioShop	CAS# 144-55-8
HEPES	BioShop	CAS# 7365-45-9
Bovine serum albumin (BSA)	Sigma-Aldrich	A7906
Dulbecco's Modified Eagle’s Medium (D-MEM) media, high glucose	Sigma-Aldrich	D5796
DMEM media, low glucose	Gibco	11885-084
Critical commercial assays
Mouse ultrasensitive insulin enzyme-linked immunosorbent assay (ELISA) kit	Alpco	80-INSMSU-E01
Insulin ultra-sensitive assay kit	Cisbio	62IN2PEG
Experimental models: Cell lines
Mouse: MIN6 K8	Prof. S. Seino (Kobe University, Japan) and Prof. J. Miyazaki (Osaka University, Japan)	N/A
Experimental models: Organisms/strains
Mouse: Male C57BL/6 at 8–18 weeks old	Charles River Laboratories	027
Mouse: Male CD-1 at 8–12 weeks old	Charles River Laboratories	022
Mouse: Male FVB at 8–12 weeks old	Charles River Laboratories	207
Software and algorithms
GraphPad Prism	GraphPad Software	www.graphpad.com
Other
Blood collection tubes	SARSTEDT	Microvette® CB 300 K2E
Gavage plastic feeding tubes, 20 ga × 30 mm	Instech	FTP-20-30
Millex GP filter (0.22 μm polyethersulfone membrane)	Sigma-Aldrich	SLGPR33RS
Glucometer	Ascensia Diabetes Care	Contour® NEXT
CONTOUR® NEXT blood glucose test strips	Ascensia Diabetes Care	7322
1 mL syringe	BD	309659
3 mL syringe	BD	303061
0.5 mL insulin syringe	BD	324703
30G PrecisionGlide needle	BD	305106
Surgical microscope	OLYMPUS	SZ51
Standard dissecting scissors	Fisher Scientific	08-951-20
Surgical clamps	Fisher Scientific	16-100-117
Blunted forceps	Fisher Scientific	10-285
Pointed forceps	Fisher Scientific	12-000-131
Surgical thread	Fine Science Tools	18020-00
384-well plate	Greiner Bio	784075
Pherastar machine	BMG – Labtech	n/a