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. 2022 Feb 19;41:69. doi: 10.1186/s13046-022-02285-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — DICER1-AS1 inhibits glycolysis, proliferation and metastasis of PC cells. A Real-time PCR analysis of DICER1-AS1 level in BxPC-3 and PANC-1 cells after transfected with the pcDNA-DICER1-AS1 plasmid (DICER1-AS1) and negative control (Vector), respectively. B The proliferative capability was performed by MTT assay in BxPC-3 and PANC-1 cells transfected with pcDNA-DICER1-AS1 (DICER1-AS1) or empty vector control (Vector). C Migration ability was assessed by wound healing assay in BxPC-3 and PANC-1 cells transfected with pcDNA-DICER1-AS1 (DICER1-AS1) or empty vector control (Vector). D Transwell assay was applied to assess the invasion ability of those cells transfected indicated above. E The change of ECAR levels in BxPC-3 and PANC-1 cells transfected with vector or DICER1-AS1 (n = 3). The glucose (10 mmol·L⁻¹), oligomycin (2 μmol·L⁻¹), or 2-deoxyglucose (2-DG, 50 mmol·L⁻¹) were used at indicated points. F Seahorse extracellular flux assay showing the oxygen consumption rate (OCR) in BxPC-3 and PANC-1 cells transfected with vector or DICER1-AS1 (n = 3). G The glucose uptake levels in BxPC-3 and PANC-1 cells transfected with control or DICER1-AS1 vectors (n = 3). H The relative lactic acid levels were detected in BxPC-3 and PANC-1 cells transfected with control or DICER1-AS1 vectors (n = 3). I The ATP levels in BxPC-3 and PANC-1 cells transfected with control or DICER1-AS1 vectors (n = 3). All data were presented as means ± SD of at least three independent experiments. Values are significant at ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 as indicated