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. 2022 Feb 19;41:69. doi: 10.1186/s13046-022-02285-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — DICER1 is a critical target for DICER1-AS1 regulating glycolysis. A Schematic illustration showing the genomic location of DICER1-AS1 and DICER1. B The expression correlation between DICER1-AS1 and DICER1 in PC tissues derived from the R2 database (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi). C Kaplan–Meier survival analysis of PC patients with high or low levels of DICER1 (cutoff value = 2886.20) derived from the TCGA database. D-E GO and KEGG analyses of DICER1-correlated genes in PC tissues from GEO database (GSE16515). F Real-time qRT-PCR assay (left panel) and western blot assay (right panel) showing the expression of DICER1 in BxPC-3 and PANC-1 cells transfected with control or DICER1-AS1 plasmids. (n = 3). G RNA-FISH and immunofluorescence detection of DICER1-AS1 (red) and DICER1 (green) in DICER1-AS1 overexpressed cells (BxPC-3). H The DICER1 mRNA half-life (t_1/2) was detected by real-time PCR in BxPC-3 cells transfected with control vector or DICER1-AS1 plasmids. I Schematic illustration of luciferase reporter constructs used for examining the effects of DICER1-AS1 on the DICER1 promoter (upper panel). The dual-luciferase assay showing the relative activity of DICER1 promoter in BxPC-3 and PANC-1 cells transfected with control vector or DICER1-AS1 (lower panel). J Real-time qRT-PCR (left panel) and western blot assay (right panel) showing the change of transcript and protein levels of DICER1 in BxPC-3 and PANC-1 cells transfected with control or DICER1-AS1 plasmids and those co-transfected with siNC or siDICER1. K-L The relative glucose uptake levels and lactic acid production were detected in BxPC-3 and PANC-1 cells transfected with control or DICER1-AS1 plasmids and those co-transfected with siNC or siDICER1. All data were presented as means ± SD of at least three independent experiments. Values are significant at ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 as indicated