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. 2022 Feb 19;41:69. doi: 10.1186/s13046-022-02285-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — DICER1-AS1 binding protein YY1 is essential for the expression of DICER1. A The diagram for selected candidate transcription factors from the TCGA and GEO (GSE21501) database using the catRAPID and JASPAR website. B The heatmap showing the prediction of the interaction between DICER1-AS1 and YY1 protein. The colors of the heatmap showing the interaction score (ranging from -3 to 3) of the individual amino acid and nucleotide pairs. The interaction propensity between DICER1-AS1 and YY1 protein is 47 and the discriminative power is 93%. C The expression correlation between DICER1-AS1 and DICER1 in PC tissues derived from the GEO database (GSE21501) and the TCGA database. D Kaplan–Meier survival analysis of patients with high or low levels of YY1 in PC patients derived from the GEO database (cutoff value = 5.81). E RIP assay was applied using the anti-YY1 antibody and IgG antibody. The detection of DICER1-AS1 (left panel) was performed using specific primers in BxPC-3 and PANC-1 cells. Then the PCR products were run on a 2% agarose gel (right panel). F Western blot of the proteins from RNA pull-down assays using anti-YY1 and anti-HuR antibodies. RNA from the 3´ untranslated region (UTR) of the androgen receptor (AR) served as the control, containing UC-rich HuR binding regions. G Western blot of YY1 pulled down by full-length (F1: 1–830) or a series of DICER1-AS1 truncates or antisense in BxPC-3 cells. H DICER1 mRNA (upper panel) and protein (lower panel) were detected in BxPC-3 and PANC-1 cells transfected with empty vector, DICER1-AS1, antisense or DICER1-AS1 truncates. I RIP assay (right panel) was performed in BxPC-3 cells to detect the binding efficiencies between different fragments of YY1 protein with DICER1-AS1. Schematic structures of YY1 protein and three truncated mutants of YY1 variants were used in this study (left panel). J Visualization of interaction between the 3D structure of the YY1 zinc-finger domain and DICER1-AS1 (374–830 nt). All data were presented as means ± SD of at least three independent experiments. Values are significant at ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 as indicated