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. 2022 Feb 19;41:69. doi: 10.1186/s13046-022-02285-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — miR-5586-5p is an essential target for the DICER1-AS1/DICER1 pathway. A The flow chart for selected candidate miRNAs. B Bubble plots showing the expression correlation between DICER1-AS1 and candidate miRNAs. C Bubble plots showing the expression correlation between DICER1 and candidate miRNAs. D Boxplots showing the miR-5586-5p level in PC tissues with different statuses of stages and grades. E Real-time qRT-PCR showing the levels of candidate miRNAs in BxPC-3 and PANC-1 cells transfected with control vector or DICER1 plasmids. F RIP assays showing the binding efficiency between DICER1 protein and pre-mir-5586 using the anti-DICER1 antibody in BxPC-3 and PANC-1 cells transfected with siNC or siDICER1 (#1, #2). G RIP assays showing the binding efficiency between DICER1 protein and pre-mir-5586 using the anti-DICER1 antibody in BxPC-3 and PANC-1 cells transfected with vector control or DICER1 plasmids. H Real-time qRT-PCR showing the levels of miR-5586-5p in BxPC-3 and PANC-1 cells transfected with siNC, siDICER1-AS1, empty vector, or DICER1, and those co-transfected with empty vector, DICER1, siNC, or siDICER1. I-J The relative glucose uptake and the relative lactic acid production were detected in PC cells transfected with siNC, siDICER1-AS1, empty vector, or DICER1-AS1, and those co-transfected with miR-NC, miR-5586-5p mimics, inh-NC, or miR-5586-5p inhibitors. All data were presented as means ± SD of at least three independent experiments. Values are significant at ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 as indicated