Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 19;41:69. doi: 10.1186/s13046-022-02285-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 7 — miR-5586-5p regulates glycolysis by inducing mRNA degradation of glycolytic genes. A GSEA analysis of miR-5586-5p-correlated genes derived from PC tissues (TCGA). NES, normalized enrichment score. B The flow chart for selected candidate glycolytic genes of miR-5586-5p target genes from the TargetScan database (http://www.targetscan.org/mamm_31/), and associated with overall survival of PC patients in TCGA database, and over-lapping analysis with genes differentially expressed (P < 0.001, Fold change > 1.5) in GEO database (GSE16515, GSE41368). C Schematic illustration showing the base pairing between miR-5586-5p and the 3’UTRs of glycolytic genes (SLC2A1, HK2, PGK1, LDHA) predicted by TargetScan database. D Bubble plots showing the expression correlation between miR-5586-5p and candidate glycolytic genes in PC tissues from the TCGA database. E–F Real-time qRT-PCR (left panel) and western blot (right panel) showing the levels of candidate glycolytic genes in BxPC-3 cells transfected with miR-NC, miR-5586-5p mimics, inh-miR-NC, or inh-miR-5586-5p. G RIP assays showing the effect of miR-5586-5p on the interaction of AGO2 with candidate glycolytic genes in PC cells using anti-AGO2 antibody. IgG was used as a negative control. H Schematic illustration showing the luciferase reporter constructs used for examining the effects of miR-5586-5p on the 3’UTR (WT, MUT) of SLC2A1, LDHA, HK2, and PGK1. WT: wild type; MUT: mutant type. I The dual-luciferase assay showing the luciferase reporter activity of 3’UTR of candidate glycolytic genes in BxPC-3 cells transfected with miR-NC, miR-5586-5p mimics, inh-miR-NC or inh-miR-NC, and those co-transfected with siNC, siDICER1-AS1, empty vector, or DICER1-AS1. J-K qRT-PCR and western blot showing the levels of glycolytic genes in BxPC-3 cells transfected with miR-NC, miR-5586-5p mimics, inh-miR-NC, or inh-miR-NC, and those co-transfected with siNC, siDICER1-AS1, empty vector, or DICER1-AS1. All data were presented as means ± SD of at least three independent experiments. Values are significant at ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 as indicated