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. 2022 Feb 19;20:34. doi: 10.1186/s12958-022-00912-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — GDF-11 expression levels are not varied in hGL cells and follicular fluid between normal and PCOS patients. A, and B, Follicular fluid samples were collected from 36 non-PCOS and 36 PCOS patients of similar age. The body mass index (BMI), antral follicle count (AFC), and basal levels of hormones in serum including follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P4), and testosterone (T) were measured. C, GDF-11 protein levels in follicular fluid samples were measured by ELISA. D, GDF-11 mRNA levels in hGL cells obtained from 12 non-PCOS and 12 PCOS patients were measured by RT-qPCR. The clinical results are expressed as the mean ± SD