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. 2022 Feb 19;41:68. doi: 10.1186/s13046-022-02272-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — A Mechanism of ZGd-NR-sensitized radiation for ICD induction and TAM depletion to synergistically boost antitumor immunity. B-K Immunogenic cell death induction and immunosuppressive tumor microenvironment reprogramming. B Immunofluorescence of CT26 cells stained with anti-CRT antibody, scale bar = 20 μm. C Quantification of relative CRT mean fluorescence intensity (n = 5 biologically independent cells). D Detection of cytoplasmic HMGB1 by ELISA kit (n = 5 biologically independent cells). E Detection of ATP secretion by luciferin-based ATP assay kit (n = 5 biologically independent cells). F Regulation of tumor immunity by TAMs. G Flow cytometry analysis of TAMs (F4/80+ and CD11b+) in tumor tissues (n = 10 biologically independent animals). H Quantification of the levels of TGF-β1 (I), IL-10 (J), and VEGF-A in tumor tissues by ELISA kit, the tumor tissues were harvested 48 h after radiotherapy (0 or 6 Gy × 1, n = 8 biologically independent animals). K Flow cytometry analysis of DCs maturation (CD80+ and CD86+ gated on CD11c+) in tumor-draining lymph nodes; the TDLNs were harvested 5 days after radiotherapy (0 or 6 Gy × 1, n = 8). All data were shown as mean ± SD. N.S. represented non-significance, *p < 0.05, **p < 0.01, ***p < 0.001 [141]