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. 2022 Jan 21;60(3):22. doi: 10.3892/ijo.2022.5312

Figure 7.

Figure 7

Targeting MUCL1 increases sensitivity towards IRI. (A) HT-29-Control siRNA and HT-29-MUCL1siRNA clones ML1 and 2 cells were treated with IRI (50 and 100 µM) for 24 h. (B) Percentage of total cell death was analyzed by Annexin V/PI staining using flow cytometry. (C) SW620-Control siRNA and SW620-MUCL1siRNA clones ML1 and 2 cells were exposed to IRI (50 and 100 µM) for 24 h. (D) Total cell death (%) was analyzed by Annexin V/PI staining using flow cytometry. Total cell death (%) shown is representative of three independent experiments (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. control. HT-ML1/HT-ML2 vs. control (black); HT-ML1-IRI-50/HT-ML2-IRI-50 vs. HT-IRI-50 (blue). SW-ML1/SW-ML2 vs. control (black); SW-ML1-IRI-50/SW-ML2-IRI-50 vs. SW-IRI-50 (blue); SW-ML1-IRI-100/SW-ML2-IRI-100 vs. SW-IRI-100 (red). MUCL1, mucin-like 1; si-, small interfering; Con, control.