TABLE 1.
Primers used in the present study
| Primer designation | Targeta | Similarityb | Probe no.c | Sequence | Product size (bp) | Annealing temp (°C) |
|---|---|---|---|---|---|---|
| 1299-1 | ECs1299 | Integrase | 1 | 5′-TAAGGCAGTGGTTATCGACG-3′ (20-mer) | 801 | 63 |
| 1299-2 | 5′-ATGCCTGCATCATCGGTACA-3′ (20-mer) | |||||
| 1312-1 | ECs1312 | TraT | 2 | 5′-GGAATTCTTGAGATTTTCTACAATCG-3′ (26-mer) | 759 | 50 |
| 1312-2 | 5′-GTCGACTCAGAGTATATTGGCGATT-3′ (25-mer) | |||||
| 1324-1 | ECs1324 | UreC | 3 | 5′-TCTAACGCCACAACCTGTAC-3′ (20-mer) | 397 | 60 |
| 1324-2 | 5′-GAGGAAGGCAGAATATTGGG-3′ (20-mer) | |||||
| 1360-1 | ECs1360 | Iha | 4 | 5′-ATGATAACCGGGATGGGCAA-3′ (20-mer) | 964 | 58 |
| 1360-2 | 5′-ATGATGCCACCTCTTCGGTG-3′ (20-mer) | |||||
| 1391-1 | ECs1391 | BfpM | 5 | 5′-GAACAGGGAAATTCAGCAGC-3′ (20-mer) | 475 | 60 |
| 1391-2 | 5′-ATCGACGATTGCTGGAAAGG-3′ (20-mer) | |||||
| 1396-1 | ECs1396 | Fluffing protein (AIDA-1) | 6 | 5′-ACTGGTTACCAGTACTGCTG-3′ (20-mer) | 883 | 60 |
| 1396-2 | 5′-ACCAGTCTTCATCGCTGTCA-3′ (20-mer) | |||||
| 1409-1 | ECs1409 | L0010 | 7 | 5′-ATATCACAATCTCCCGTCCG-3′ (20-mer) | 790 | 63 |
| 1409-2 | 5′-AGTCTGTCAACCAGTTCTGG-3′ (20-mer) |