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. 2021 Aug 29;29(6-7):e12726. doi: 10.1111/micc.12726

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© 2021 The Authors. Microcirculation published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Live imaging of Weibel‐Palade Body trafficking in 2D culture and 3D sprouting. (A) Live imaging of endothelial cells expressing GFP‐Rab27a (Weibel‐Palade Body marker) over time in 2D culture. Red arrowheads denote puncta accumulated at the leading edge of the cell. (B) Live imaging of fibrin bead generated sprout expressing GFP‐Rab27a over time. Red arrowheads indicate accumulations of Weibel‐Palade Bodies at cell‐cell interface. White boxes are areas of magnification and red botted lines indicated cell boundaries. L denotes lumen [Colour figure can be viewed at wileyonlinelibrary.com]