. Author manuscript; available in PMC: 2022 Feb 20.

Published in final edited form as: Nature. 2021 Oct 13;598(7882):646–651. doi: 10.1038/s41586-021-04013-0

Extended Data Table 2.

Overview of Mouse Lines and Experiments

Mouse Line	Behavior	Approach	Virus
Oprm1 KO	Sucrose Consumption (Figure 1)	N/A	N/A
	Sucrose Consumption (Figure 3)	Viral Injection	AAV5-EF1α-DIO-rMOR-GFP
	Morphine CPP (Figure 3)	Viral Injection	AAV5-EF1α-DIO-rMOR-GFP
Oprm1 KO x Penk-Cre	Sucrose Consumption (Figure 3)	Viral Injection	AAV5-EF1α-DIO-rMOR-GFP
Oprm1^fl/fl	Sucrose Consumption (Figure 1)	Viral Injection Viral Injection Viral Injection	rAAV5-CMV-Cre-GFP AAV2retro-GFP-Cre AAV2retro-CMV-myc-NLS-Cre
Pdyn-Cre x Oprm1^fl/fl	Sucrose Consumption (Figure 1)	Genetic Cross	N/A
Penk-Cre x Oprm1^fl/fl	Sucrose Consumption (Figure 1)	Genetic Cross	N/A
Penk-IRES-Cre	Anatomical Tracing (Figure 2)	Viral Injection	AAV2retro-EF1α-DIO-EYFP
	Sucrose Solution Consumption (Figure 2)	Viral Injection, Fiber Photometry	AAV5-DJ-EF1α-DIO-GCAMP6s
	Sucrose Solution Consumption (Figure 3)	Viral Injection, Optogenetics	AAV5-EF1α-DIO-oMOR-EYFP
	Sucrose Consumption (Figure 4)	Viral Injection, 1-Photon Imaging	AAV5-DJ-EF1α-DIO-GCAMP6s
	Sucrose Consumption (Figure 5)	Viral Injection, Caspase	AAV2-FLEX-taCas3-TEVp
	Sucrose Consumption (Figure 5)	Viral Injection, CRISPR	AAV1-CMB-FLEX-Sa-Cas9U6-sgPenk
	Sucrose Pellet Consumption (Figure 5)	Viral Injection, Fiber Photometry	AAV5-DJ-EF1α-DIO-GCAMP6s AAV1-CMB-FLEX-Sa-Cas9U6-sgPenk