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. 2022 Feb 19;13:73. doi: 10.1186/s13287-022-02744-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 14 — Immunohistochemistry staining was employed to detect the expression of RUNX2 in the bone defect area. The black arrowheads indicate RUNX2-positive osteoblasts at the MBCP–new bone interface. The red arrows indicate a high expression region and RUNX2-positive osteocytes embedded within the bone and connective tissue matrix. Scale bar = 100 μm. (NB new bone, CT connective tissue, BM bone marrow, MBCP scaffold, DPSCs dental pulp mesenchymal stem cells, Mel melatonin)