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. 2022 Feb 19;13:73. doi: 10.1186/s13287-022-02744-z

Fig. 5.

Fig. 5

Effects of melatonin (0, 100, 10, and 1 μM) on mineralization of DPSCs at 7 and 14 days after incubation with and without osteogenic differentiation medium. a, c Extracellular calcium deposition was determined with Alizarin red S staining. b, d Extracellular calcium deposition was quantified colorimetrically. Melatonin at 100 μM significantly enhanced mineralization of DPSCs with osteogenic differentiation medium. Scale bar = 100 μm. (GM growth medium, OM osteogenic medium, Mel melatonin) (*p < 0.05, **p < 0.01)