Table 1.

Primers used for RT-PCR

Gene	Type	Primers	Accession	Product length
ALP	Forward	5′-ACTGTGGACTACCTCTTG-3′	XM_017346489	76
	Reverse	5′-GGTCAGTGATGTTGTTCC-3′
BMP-2	Forward	5′-CGTGAGGATTAGCAGGTCTTTG-3′	NM_001082650	127
	Reverse	5′-TTTCGCTTGACGCTTTTCTC-3′
Osteocalcin	Forward	5′- ACTCTTGTCGCCCTGCTG-3′	XM_002715383	116
	Reverse	5′-CTGCCCTCCCTCTTGGAC-3′
Runx2	Forward	5′-TCAGGCATGTCCCTCGGTAT-3′	XM_017345160	54
	Reverse	5′-TGGCAGGTAGGTATGGTAGTGG-3′
GAPDH	Forward	5′-GCCTGGAGAAAGCTGCTAAGT-3′	NM_001082253	133
	Reverse	5′-GAGTGGGTGGCACTGTTGAA-3′