Table 2.

Summary of diagnostic uses for main Tryanosoma species responsible for animal African trypanosomosis and non-tsetse transmitted animal trypanosomosis

Target	Techniquea	Subgenus Nannomonas	Subgenus Duttonella	Subgenus Trypanozoon
		Trypanosoma congolense	Trypanosoma vivax	Trypanosoma brucei brucei	Trypanosoma evansi	Trypanosoma equiperdum
Parasite	Wet blood films	+b	+	+	+ Trypanozoon
	HTC/BCT	+  +  +	+  +  +	+  +  +	+  +  + Trypanozoon
	mAECT	−	−	−	−	−
	Inoculation of rodents	+	0	+	+  +	+
DNA	Pan-species primers (ITS1)	+  +	+  +	+  +	+  + Trypanozoon	+
	Genus/species/type-specific primers	+  +  +	+  +  +	+  +  +	+  +  + Trypanozoon	+  +
	Specific primers among Trypanozoon			0	+	0
Antibodies	ELISA on WCLSA	+  +  +	+  +  +	+  +  +	+  +  + Trypanozoon	+
	Protein-specific ELISA	Under test	Under test	0	+	0
	Agglutination test (CATT)	0	0	+	+  +  +	0
	IFAT	−	−	−	−	+
	CFT	0	0	0	0	+  +
	TL	0	0	0	+	0

^aSee Abbreviation List for the full description of each abbreviation

^bQualitative uses: +  +  +  = well adapted and used; +  +  = adapted and used; +  = can be used; 0 = not used in practice