Fig. 5.

BCL2 mutation at S70, not S87, inhibited the autophagic activity of OCPs. A OCPs transfected with WT BCL2 and the two mutant BCL2 were treated with RANKL for 8 h together with or without lysosomal protease inhibitor (E64d plus pepstatin A) in α-MEM with 2.5% FBS. LC3 protein level was detected using western blotting assays. LC3 conversion rate is expressed as the ratio of LC3II to LC3I. B The transfected OCPs were treated with RANKL for 24 h in α-MEM with 2.5% FBS. The autolysosomes (red arrows) in OCPs were observed under TEM. Scale bar, 5 μm or 1 μm. C The histograms showing quantitative results of autolysosomes in B (75 cells from 3 independent experiments). The experiments were replicated at least three times. Data are presented as the mean ± SEM from three independent experiments. ***P < 0.001. ns not significant, E E64d, P pepstatin A