Fig. 5.

CuB reversed TGF-β1-induced EMT in A549 cells through suppressing the ROS production. a Inhibition effect of CuB (15 nM) or NAC (10 mM) on the morphology changes in TGF-β1-induced A549 cells at 48 h (Scale bar = 25 μm). b The inhibition effect of CuB (15 nM) and NAC (10 mM) on the fluorescence of ROS in TGF-β1-induced A549 cells after treatment for 48 h (Scale bar = 25 μm). c The effect of CuB (15 nM) and NAC (10 mM) on the ROS level in TGF-β1-induced A549 cells for 48 h, and then treatment with DCFH₂DA (5 μM) for 30 min, and detected by flow cytometry. d The quantification of c, ^###P < 0.001 vs control group, ^***P < 0.001 vs TGF-β1 group. e Western blotting assay was used to detect the proteins expression of ZEB1, E-cadherin, N-cadherin, and Vimentin expression in TGF-β1-induced A549 cells after co-treated with CuB (15 nM) or NAC (10 mM) for 48 h. f The statistic results of protein expression of e, ^###P < 0.001 vs control group, ^***P < 0.001 vs TGF-β1 group