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. 2022 Feb 19;21:27. doi: 10.1186/s12934-021-01736-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Comparative and quantitative analysis of pigments, starch, and lipid of cells cultured under nitrogen-starvation medium (TAP-N). A Pigment contents in the wild type and mutants measured by high performance liquid chromatography (HPLC). Lut lutein; Zea zeaxanthin. B Starch content in the wild type and mutants measured by the enzymatic hydrolysis method. C Total lipid content in the wild type and mutants measured by the chloroform/methanol extraction method. The data shown are representative of three independent experiments (n > 9). Error bars indicate standard deviation. Asterisks indicate statistically significant differences based on Student’s t test (P < 0.05). ^a−cIn a row without a common superscript letter, differ (P < 0.05) as analyzed by two-way ANOVA and the Tukey test. (n = 6)