Fig. 4.
Strategic modification of the cultivation process and optimization of culture medium. A Diagram of existing oil-induction process called the two-step induction process. This process includes a medium change step, and the duration of the entire process is 6 days. B Diagram of simplified oil-induction process called the one-step induction process. The duration of the oil induction process was reduced by the lack of the medium change process by using a modified culture medium (1/4NP2A medium). C Comparison of lipid productivity between the two lipid-induction processes. D Comparison of pigment productivity between the two oil induction processes. The data shown are representative of three independent experiments (n = 9). a−cIn a row without a common superscript letter, differ (P < 0.05) as analyzed by two-way ANOVA and the Tukey test (n = 6)