Fig. 4.

FAM83A overexpression promotes cell proliferation. A Representative images of either mGFP-positive primary epithelial cells (N = 3) or paired hTERT-immortalized cells after lentiviral infection to express either control mock (CTR), FAM83A (FAM83A), pLKO control (shCTR), or shRNA for FAM83A (shFAM83A). 20 × magnification images are shown and mGFP is in green. Level of FAM83A expression in either primary (B) or immortalized (C) cells infected with either control, FAM83A-overexpression, or shFAM83A lentivirus particles. Cell viability and cell proliferation of primary (D and E) or immortalized (F and G) cells measured with SRB assay or BrdU assay, respectively. Data are shown as percentage of either cell viability or cell growth of the FAM83A overexpressing/downregulating cells versus the control cells. H Expression of cell proliferation-, apoptosis-, differentiation-, and stemness-related genes in primary epithelial cells overexpressing FAM83A (n = 3) as compared to mock-expressing cells (n = 3). Data are shown as mean ± standard error of the mean (SEM). *p < 0.05, **p < 0.001