Figure 6.

Flow cytometric analysis of splenic and renal T cells in Rag-1^−/− females following normotensive splenocyte (NS) or hypertensive splenocyte (HS) transfer and angiotensin II (ANG II) infusion. No significant difference was found in the frequency of CD4⁺ or CD8⁺ splenic (A) or renal (C) T cells regardless of donor splenocyte (NS vs. HS) or ANG II infusion (control vs. control + ANG). However, females that received HS + ANG had a significantly lower frequency of splenic FoxP3⁺ T regulatory cells compared with those that received NS + ANG (B). Interestingly, transfer of HS alone increased renal FoxP3⁺ T regulatory cells compared with NS transfer, but no impact of ANG II was seen (D). E: representative flow cytometric gating; n = 3−4 mice/group. #P < 0.05 via two-way ANOVA with a Tukey’s multiple comparison test. Rag-1, recombination-activating gene-1; FSC-A, forward scatter area; SSC-A, side scatter area.