FIGURE 4.

Osteocyte regulation of bone formation via Sclerostin production. A: Sclerostin immunostaining in paraffin-embedded human bone. Black arrow points to Sclerostin-positive osteocytes; red arrow points to Sclerostin-negative osteocytes. B: micro-computed tomography (μCT) image of vertebral cancellous bone from wild-type mice (WT) and mice with global deletion of SOST (SOST KO). C: toluidine blue-tartrate-resistant acid phosphatase (TRAP)-stained histological image of tibial cancellous bone from WT and SOST KO mice. D: dynamic histomorphometry in plastic-embedded bones from WT mice and mice overexpressing human SOST under the control of the DMP1-8kb promoter (Dmp1-8kb-hSOST). Green calcein labels and red alizarin labels are shown. E: toluidine blue-TRAP stained histological image of bones from C57BL/6 mice treated with IgG (control) or neutralizing anti-Sclerostin antibody (anti-Sclerostin-Ab). Yellow arrows point to osteoblasts on the bone surface.