Figure 6.

A: TCS-A increases KCa2.3 expression in a concentration-dependent manner in HUVECs. B: average response for five experiments normalized to KCa2.3 in the absence of TCS-A are shown. C: a time course for TCS-A-dependent increase in KCa2.3 expression in HUVECs is presented. TCS-A was used at 1 µM tubulin was used as a loading control. D: average response for three experiments normalized to KCa2.3 at T = 0 h represented as a scatter plot. An asterisk indicates significant increase in KCa2.3 expression compared with control (P < 0.05). HUVECs, human umbilical vein endothelial cells; KCa2.3, calcium-activated potassium channel; TCS-A, trichostatin-A.