Figure 2.

Cytokine and chemokine signaling in 4-wk-old myeloid mineralocorticoid receptor knockout (MRcko) mdx skeletal muscles and MRcko wild-type (WT) tibialis anterior muscles (TAs) 4 days after acute injury (AI). Proteome profiler cytokine array immunoblots incubated with lysates from 4-wk-old (4WK) MRcko mdx quadriceps (QUAD; 5 mg of protein; n: MRcko = 3 M, 3 F; Cre⁻ = 3 M, 3 F) and diaphragms (DIA; 2.5 mg of protein; n: MRcko = 3 M, 3 F; Cre⁻ = 3M, 3 F) (A) as well as with MRcko WT TAs (1.5 mg of protein; n: MRcko = 3 M, 3 F; Cre⁻ = 3 M, 3 F) 4 days after acute injury (B) compared with the genotypic Cre⁻ controls. Blots are labeled with coordinates that specifically correspond to changes in chemokine and cytokine expression quantified by densitometry in Table 1 and Table 2, respectively.