Table 2.

Cytokine quantification in MRcko mdx and WT skeletal muscle

	TNF-α	IL-1β	M-CSF	C5/C5a	IL-4	IL-10	sICAM-1	TIMP-1	IL-1α	IL-1ra
Coordinate	D3	A10	C4	A2	B2	B6	A7	D2	A9	A11
MRcko
mdx 4WK QUAD	+++	+++	↑2.8×	↑1.2×	+++	+++	↓1%	↑1.2×	+++	↓6%
mdx 4WK DIA	↓20%	↓64%	↓10%	↓1%	↓45%	↓50%	===	===	↓50%	↓11%
WT AID4 TA	↑1.6×	N.D.	↑1.3×	↑1.1×	N.D.	N.D.	===	↑1.1×	N.D.	===

Quantification of relative cytokine levels detected in myeloid mineralocorticoid receptor knockout (MRcko) mdx and injured MRcko wild-type (WT) skeletal muscle by pixel densitometry. Results are displayed as fold increases and % decreases in MRcko skeletal muscle relative to the appropriate Cre⁻ controls. Coordinates listed in the table correspond to the individual chemokine’s location (in duplicate) on the immunoblot. Some quantified cytokines were equivalent (===) or not detected (N.D.) compared with Cre⁻ controls. Other cytokines were upregulated (+++) but, however, undetected in Cre⁻ controls and therefore excluded from quantification. AID4, 4 days after acute injury; DIA, diaphragm; M-CSF, macrophage colony-stimulating factor; QUAD, quadriceps; sICAM-1, soluble ICAM-1; TA, tibialis anterior; TIMP-1, tissue inhibitor of metalloproteinase-1; 4WK, 4 wk of age.