Figure 4.
SARS-CoV-2 induces significantly worse lung histological damage than IAV
(A) One-month BCG-vaccinated and nonvaccinated (PBS) C57BL/6J mice were i.n. infected with a sublethal or lethal dose of IAV-PR8. Representative images of lung histopathology on days 0, 3, and 6 post IAV infection (left) and scoring (right), n = 4–5/group, refer to Table S1.
(B) One-month BCG-vaccinated and nonvaccinated (PBS) K18-hACE2 mice were i.t. infected with 4,000 TCID SARS-CoV-2/SB2. Representative images of lung histopathology on days 1, 3, and 5 post SARS-CoV-2 infection (left) and scoring (right), n = 3–4/group, refer to Table S1.
(C) BCG-vaccinated and control Syrian golden hamsters were i.n. infected with 1 × 105 PFU SARS-CoV-2 and exhibited a clinically mild disease phenotype. Representative images of lung histopathologies on days 0, 3, and 5 post SARS-CoV-2 infection (left) and scoring (right), n = 3–6/group, refer to Table S1.
(D) RNAscope analysis of SARS-CoV-2 viral particles in human lung cells. Heatmap data are displayed as mean. See also Figure S4 and Table S1.