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IHZ-1 caused apoptosis through activating ROS/JNK cascade in HCC cells. (A) Cells were incubated with IHZ-1 and pretreated with NAC or SP600125. Immunoblots of p-JNK and JNK protein expression was quantified. (B) The apoptosis cells were evaluated by flow cytometry using Annexin-V/PI double staining. ***p < 0.001.
