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. 2022 Feb 7;13:820685. doi: 10.3389/fimmu.2022.820685

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Copyright © 2022 Wang, Deng, Sun, Zhao, Li, Yang, Yuan, Liu, Qian, Zhou and Kang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Levels of inflammatory cytokines and proportions of Th cell subpopulations in the spleen suspensions of rats in each group. (A-I) Levels of inflammatory cytokines in the spleen suspensions of rats in each group (compared with the model group); (J) circle-gating strategy for flow cytometry analysis of the proportion of splenic Th cells; (K, L, N, O) flow analysis of the proportion of Th1, Th2, Th17, and Treg cells in the spleen as a percentage of CD4⁺ T cells; (M) the ratio of Th1/Th2 in the spleen of rats in each group; (P) the ratio of The ratio of Th17/Treg in spleen of each group. n=5, *p<0.05, **p<0.01, ***p<0.001.