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. 2022 Feb 7;13:820685. doi: 10.3389/fimmu.2022.820685

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Copyright © 2022 Wang, Deng, Sun, Zhao, Li, Yang, Yuan, Liu, Qian, Zhou and Kang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Levels of inflammatory cytokines in cell supernatants and the proportion of Th cell subpopulations in lymphocytes in each group of in vitro experiments. Lymphocytes isolated from the spleen were co-cultured with MSCs at a ratio of 10:1, and the lymphocytes and supernatants were analyzed at 6h, 24h and 72h, respectively. (A–I) Levels of inflammatory cytokines in cell supernatants of each group (*compared with the model group); (J) circle gating strategy for flow cytometry analysis of the proportion of Th cells in lymphocytes of each group; (K, L, N, O) flow analysis of the proportion of Th1, Th2, Th17, and Treg cells in lymphocytes of each group as a percentage of CD4⁺ T cells; (M) ratio of Th1/Th2 in lymphocytes of each experimental group; (P) ratio of Th17/Treg in lymphocytes of each experimental group. n=5, *p<0.05, ***p<0.001.