Figure 4: Upregulated EV-miRNA associated with heightened inflammatory response in PBMC.
A) Experimental design for miRNA candidate selection and electroporation created using Biorender.com. B,C) PBMC from control macaques were transfected with miRNA mimics and further stimulated with PMA/ionomycin. The production of TNFα (D) and IL-6 (E) was measured from supernatants by ELISA. P-value significance is denoted by *=<0.05, **=<0.01, ***=<0.001.