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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Adv Biol Regul. 2021 Nov 27;83:100842. doi: 10.1016/j.jbior.2021.100842

Figure 1. The flexible SNX1 N-terminus binds full-length SNX27 in vitro.

Figure 1.

(A) Domain schematics of SNX27 and SNX1. SNX27 contains an N-terminal PDZ domain (light blue), middle PX domain (grey), and C-terminal FERM-like domain (purple). The FERM domain is further divided into F1, F2, and F3 sub-domains (not shown). SNX1/SNX2 contains a flexible N-terminal region followed by PX and BAR domains. (B) Pulldown assays were conducted using purified SNX27 and SNX1 proteins; Western blots were probed with anti-His (Abcam). The left-hand blot shows GST-tagged full-length SNX27 as bait and His-tagged SNX1 constructs (see text) as prey. GST-SNX27 pulls down full-length SNX1, but not constructs containing either SNX1 PX-BAR or SNX1 BAR domain alone. The right-hand blot demonstrates that GST-SNX1 (residues 1-142) pulls down full-length SNX27. (C) Summary of pulldown binding data.