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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Adv Biol Regul. 2021 Nov 27;83:100842. doi: 10.1016/j.jbior.2021.100842

Figure 2. Two short regions in SNX1 mediate binding to SNX27.

Figure 2.

(A) Biochemical mapping experiments define two regions in the SNX1 N-terminus that bind full-length SNX27. Purified GST-SNX1 and H6-SNX27 proteins were used in pulldown experiments; a Western blot probed with anti-His is shown. Three GST-tagged SNX1 constructs exhibit robust binding to full-length H6-SNX27: GST-SNX1 (residues 1-90); GST-SNX1 (residues 40-90); and GST-SNX1 (residues 40-100). (B) Schematic summary of SNX1/SNX27 pulldown binding data.