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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Allergy Clin Immunol. 2021 Aug 21;149(3):1044–1059.e5. doi: 10.1016/j.jaci.2021.07.041

Figure 1.

Figure 1.

TLR3 agonist poly (I:C) inhibits Alternaria alternata-induced innate type 2 response. (A) Experimental model for Panel B. (B) Lung levels of type 2 cytokines were analyzed by ELISA. *p<0.05 compared with mice pretreated with PBS and exposed i.n. to A. alternata. Data are presented as the mean ± SEM (n = 2 in each group). (C) IL-5venus mice were pretreated with poly (I:C) or PBS i.n., and exposed 24 h later to A. alternata or PBS. At 6 h, lung single-cell suspensions were analyzed by gating on Lin−C25+CD44high lung ILC2s. Representative scattergrams are shown. (D) Total number of ILC2s, proportion of IL-5venus-positive ILC2s and mean fluorescence intensity (MFI) of IL-5venus in ILC2s are presented. **p<0.01 between the groups is indicated by horizontal lines. Data are presented as the mean ± SEM (n = 2-3 in each group) and are a pool of two experiments. (E) Experimental model for Panels F and G. (F) Cell numbers and differentials in BAL fluids were analyzed. (G) Lung levels of type 2 cytokines were analyzed by ELISA. *p<0.05, **p<0.01 between the groups indicated by horizontal lines. Data are presented as the mean ± SEM (n = 4 in each group).