Figure 2.
The inhibitory effects of poly (I:C) is mediated by type 1 IFNs. (A) Naïve BALB/c mice were administrated poly (I:C), R848, or CpG A i.n.. After 6 h, lung levels of interferons were analyzed by ELISA. The intact group indicates naïve mice without any manipulations. *p<0.05, **p<0.01 compared to mice administered PBS. Data are presented as the mean ± SEM (n = 3 in each group). (B) Naïve BALB/c mice were administrated poly (I:C) or PBS. Kinetic changes in the lung levels of interferons were analyzed. *p<0.05, **p<0.01 compared to mice administered PBS. Data are presented as the mean ± SEM (n = 3 in each group). (C) Experimental model for Panels D and E. (D, E) The results with Ifnar1−/− and Ifngr1−/− mice are presented in Panel D and Panel E, respectively. Data are presented as the mean ± SEM (n = 5 in each group) and are representative of two experiments. **p<0.01 between the groups indicated by horizontal lines. (F) Experimental model for Panel G. (G) Lung levels of type 2 cytokines are presented. *p<0.05, **p<0.01 between the groups indicated by horizontal lines. Data are presented as the mean ± SEM (n = 3 in each group) and are representative of three experiments.