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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Allergy Clin Immunol. 2021 Aug 21;149(3):1044–1059.e5. doi: 10.1016/j.jaci.2021.07.041

Figure 5.

Figure 5.

IFN-β induces apoptosis of lung ILC2s. (A) Isolated lung ILC2s were cultured with medium alone, IL-33, or IL-7 with or without IFN-β for 72 h. Cell viability was analyzed by staining with Annexin-V and 7-AAD. (A) Representative scattergrams are shown. (B) Proportions of live (Annexin V7-AAD), apoptotic (Annexin V+7-AAD), and necrotic (Annexin V+7-AAD+) cells are shown. Data are presented as the mean ± SEM (n = 2) and are representative of three experiments. **p<0.01 between the groups indicated by horizonal lines.