Figure 5.
IFN-β induces apoptosis of lung ILC2s. (A) Isolated lung ILC2s were cultured with medium alone, IL-33, or IL-7 with or without IFN-β for 72 h. Cell viability was analyzed by staining with Annexin-V and 7-AAD. (A) Representative scattergrams are shown. (B) Proportions of live (Annexin V−7-AAD−), apoptotic (Annexin V+7-AAD−), and necrotic (Annexin V+7-AAD+) cells are shown. Data are presented as the mean ± SEM (n = 2) and are representative of three experiments. **p<0.01 between the groups indicated by horizonal lines.