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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Allergy Clin Immunol. 2021 Aug 21;149(3):1044–1059.e5. doi: 10.1016/j.jaci.2021.07.041

Figure 6.

Figure 6.

IL-7 induces GATA3 mRNA and protein expression in lung ILC2s. (A) Isolated lung ILC2s were cultured with medium alone or with IL-7 for 16 h. mRNA was analyzed by the Nanostring® assay. (A) The results of unsupervised heat map analysis are shown. Sample numbers indicate a paired and separate experiment. (B) Scatter plots of all the analyzed genes are shown. Red dots indicate notable genes. (C) Isolated lung ILC2s were cultured with medium alone or with IL-7 for 16 h. mRNA was analyzed by real-time RT-PCR, and the results were normalized to the cells cultured with medium alone. (D) Isolated lung ILC2s were cultured with medium alone or with IL-7 for 72 h. Cells were stained with Ghost Dye Red 780, followed by staining for GATA3 protein. Cells were analyzed by gating separately on live cells (Ghost Dye Red 780-negative) and dead cells (Ghost Dye Red 780-positive). Representative scatter grams and histograms are shown.